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High molecular weight D204 resin to heparin sodium has the characteristics like: big exchange capacity, easy elution, stable physicochemical properties, fast exchange speed, high mechanical strength, strong organic pollution resistance, long use life, etc. It is widely used in the extract of heparin sodium.

一、Summarize

D204 resin is strong base anion exchange resin. Since it has specific pore structure, it has the characteristics like: big exchange capacity, easy elution, stable physicochemical properties, fast exchange speed, high mechanical strength, strong organic pollution resistance and long use life to high molecular weight heparin sodium. It is widely used in the extract of heparin sodium. Heparin has obvious anticoagulant action. It has the characteristics like: easy to handle, low cost and high benefit.

二、Quality index of D204 resin(Executive standard：QY/JH0267-1998)

三、Performance of D204 resin

Heparin is a acid mucopolysaccharide. It has many anionic groups on polysaccharide chain. At the same time, the molecular weight of heparin is big, so the resin should have enough big apertures. Now, D204 resin is macro-reticular typeion exchange resin, its pore structure aperture, ratio and surface area parameter is the most suitable for macromolecular heparin adsorption and elution. D204 resin is the most suitable for ion exchange method extracting heparin sodium.

四、Matters need attention

1、Intestinal mucosa quality:

The quality of the intestinal mucosa directly influence on product yield and valence. Must use fresh intestinal mucosa.

2、Effects of temperature and water quality:

The different climates and water quality conditions have different affect to resin's exchange ability. Pay highly attention water quality attention, because the ion in water can severely reduce the purity of heparin. Therefore, it is better to use the tap water that is precipitation treated. The conditional place or unit had better use distilled water or deionized water.

3、Use proportion of resin:

Through test, use D204 resin absorbs heparin, the dosage is better to controlled as 3%. Too much or too little resin will affect the effective yield of heparin.

4、Storage of new resin:

If new resins need long-term storage, should pay attention to prevent resin dehydration. If the unsuitable keeping makes resin dry up, the resin activity will be seriously affected. Resin storage temperature had better be controlled between 5℃ and 40℃, thus to ensure the strength and life of resin.

If the already used old resin is not used temporarily, it can first be treated by 1mol/l alkali and 1mol/lHydrochloric acid, wash with water and then soak in the saturated salt water. It should be often checked in summer, if the salt water is stunk, change the salt water in time, thus to keep the quality of the resin.

五、Concentration table of commonly used reagent

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